434200

antibody from Invitrogen Antibodies

Targeting: NR5A1 AD4BP, ELP, FTZ1, FTZF1, hSF-1, SF-1, SF1

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Validation data

Product number: 434200 - Provider product page
Provider: Invitrogen Antibodies
Product name: NR5A1 Monoclonal Antibody (N1665)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: This antibody specifically recognizes human SF-1 and cross reacts with mouse and rat SF-1.
Antibody clone number: N1665
Concentration: 1 mg/mL

NR5A1 protein structure - 434200 shown in red.

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5. SF-1 and Ang2 expression during early human fetal adrenal development. Top panel: immunohistochemistry for SF-1 and Ang2 in the human fetal adrenal gland (fetal stage 1, 8 wk postconception) shows strongest expression of these factors in the developing definitive zone (DZ). FZ, fetal zone. Nuclear staining is shown with DAPI. Bottom panel: higher-power magnification shows coexpression of SF-1 and Ang2 in areas of the definitive zone.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 Anti-SF-1 staining of tumour and normal tissue. Anti-SF-1-stained sections showed strong nuclear staining in tumour tissue (A) and negative staining in normal tissue (B). Scale bar: 100 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 13. Identification of potential testis-secreted proteins. ( a ) Heatmap representing normalised gene expression values for potential secreted proteins. The intensity of gene expression is indicated by a colour scale: blue for lowest and red for highest expression levels. ( b ) Immunohistochemistry for AMH (MIS) in human fetal testis at 9 wpc. NR5A1 (SF-1) was used to highlight Leydig cells (green). DAPI was used to counterstain nuclei (blue) and to highlight the outer capsule. Scale bars, 100 um (top panels) and 20 um (bottom panels) ( c ) Immunohistochemistry for INSL3 in human fetal testis at 9 wpc. NR5A1 (SF-1) was used to highlight Leydig cells (green). DAPI was used to counterstain nuclei (blue). Scale bar, 50 um ( d ) Immunohistochemistry for SCUBE1 in human fetal testis at 9 wpc. NR5A1 (SF-1) was used to highlight Leydig cells (green). DAPI was used to counterstain nuclei (blue). Scale bars, 100 um (top panels) and 20 um (bottom panels).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1. Axin2 expressing cells contribute to pituitary growth and expansion of all lineages. ( A ) Immunofluorescence staining against GFP (green) with markers of pituitary stem cells (PSCs) or lineage commitment (magenta) in Axin2 CreERT2/+ ; ROSA26 mTmG/+ pituitaries harvested from mice induced at P14 and lineage traced for 2 days (top panel) and 14 days (bottom panel). Scale bar: 10 mum. ( B ) Quantification of lineage expansion between 2 and 14 days following induction at P14. Graph shows that the proportion of lineage committed cells (either PIT1 + , TPIT + , or SF1 + ) and PSCs (SOX2 + ), that is, that are transcription factor (TF) + cells that are GFP + increases between 2 days (black bars) and 14 days (grey bars) post-induction. PIT1 p=0.000004, TPIT p=0.008 multiple t -tests. n = 4 animals per time point. ( C ) Immunofluorescence staining against GFP (green) in pituitaries harvested from Axin2 CreERT2/+ ;ROSA26 mTmG/+ mice induced at P14 and lineage traced for 2 days, 2 weeks, and 8 weeks. Bottom panel shows magnified fields of view of regions of interest indicated by white boxes in panels above. Scale bars: 50 mum. ( D ) Top panel showing the quantification of the proportion of all cells in Axin2 CreERT2/+ ;ROSA26 mTmG/+ pituitaries that are GFP + at 2, 7, 14, 28, and 56 days post-induction as analysed by flow cytometry. Days 2-7 p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 1 Figure Histological and immunohistochemical features of selected GT. A-C, Case #1 (Group 1) shows a monomorphic tumour with diffuse, strong SF-1 and GATA3 immunoreactivity. D-F, Case #28 (Group 2) was known not to recur within 8.6 years. The tumour is histologically monomorphic (D) and shows diffuse, strong SF-1 expression throughout the tumour (E). F, Unlike case #1, this tumour has rare neoplastic nuclei that weakly label for GATA3, shown at higher magnification in inset (F'). G-I, Case #34 (Group 3) recurred within 3.8 years after initial resection. SF-1 shows immunopositivity in a perivascular distribution with abrupt negative staining in the nests (H). A similar patchy staining is noted with a GATA3 immunostain (I). J-M, Case #35 (Group 3) recurred within 6.0 years. Clear cell change is noted in the centre of nests, whereas tumour cells with amphophilic cytoplasm are noted in a perivascular distribution. Patchy SF-1 (K) and GATA3 (L) staining is seen in the tumour. M: Immunocytologic variability of SF-1 staining within GT is shown and graded from 1-4. N: A 2 x 2 contingency table of Groups 2-4 showing that most recurrent tumours had patchy SF-1 labelling unlike in the non-recurrent group. Scale bar: 100 mum

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 3 Figure Gene expression profiling data obtained following RNA sequencing. A, Heatmap of unsupervised hierarchical cluster analysis of the 50 most significantly differentially expressed genes after variance stabilising transformation of high SLI (>80%, n = 5) and low SLI (

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 4 Figure The expression of marker genes for interstitial cells in 3-day cultures established from PDGFRA - and PDGFRA + cell populations. (a-c) Immunofluorescent staining of 6 dpp PDGFRA - (a), 6 dpp PDGFRA + (b) cultures and a testis section (c) for NR5A1, which is highly expressed in Leydig cells. (d-f) Immunofluorescent staining of 6 dpp PDGFRA - (d), 6 dpp PDGFRA + (e) cultures, and a testis section (f) for PECAM1, an endothelial cell marker. Right panels in (a-f) show merged images with DAPI- or DRAQ5-stained nuclei. (g-i) The expression levels of marker genes for Leydig cells (g), endothelial cells (h), and leukocytes (i) were low in 6 and 12 dpp PDGFRA - cultures relative to that in adult testicular tissue (the zero line). Data are presented as the mean +- SEM from three independent experiments. * p < 0.05 and ** p < 0.01. Scale bars: 200 mum (a,d) and 50 mum (b,c,e,f). dpp, days postpartum; PDGFR, platelet-derived growth factor receptor alpha and beta.