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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF2554 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD200R1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse CD200 R1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human CD200 R is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
BAE42266- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references DNER modulates the length, polarity and synaptogenesis of spiral ganglion neurons via the Notch signaling pathway.
CD200R/Foxp3-mediated signalling regulates microglial activation.
Expression of CD200 in alternative activation of microglia following an excitotoxic lesion in the mouse hippocampus.
The immunosuppressive surface ligand CD200 augments the metastatic capacity of squamous cell carcinoma.
Du J, Wang X, Zhang X, Zhang X, Jiang H
Molecular medicine reports 2018 Feb;17(2):2357-2365
Molecular medicine reports 2018 Feb;17(2):2357-2365
CD200R/Foxp3-mediated signalling regulates microglial activation.
Yi MH, Zhang E, Kim JJ, Baek H, Shin N, Kim S, Kim SR, Kim HR, Lee SJ, Park JB, Kim Y, Kwon OY, Lee YH, Oh SH, Kim DW
Scientific reports 2016 Oct 12;6:34901
Scientific reports 2016 Oct 12;6:34901
Expression of CD200 in alternative activation of microglia following an excitotoxic lesion in the mouse hippocampus.
Yi MH, Zhang E, Kang JW, Shin YN, Byun JY, Oh SH, Seo JH, Lee YH, Kim DW
Brain research 2012 Oct 24;1481:90-6
Brain research 2012 Oct 24;1481:90-6
The immunosuppressive surface ligand CD200 augments the metastatic capacity of squamous cell carcinoma.
Stumpfova M, Ratner D, Desciak EB, Eliezri YD, Owens DM
Cancer research 2010 Apr 1;70(7):2962-72
Cancer research 2010 Apr 1;70(7):2962-72
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- CD200 R1 in Mouse Splenocytes. CD200 R1 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD200 R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2554) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
