Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 12-9201-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD200 Receptor Monoclonal Antibody (OX108), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody OX108 recognizes human CD200R also known as OX2. CD200R is an inhibitory receptor with a similar structure as its ligand, CD200, yet lacks an ITIM domain typically found in inhibitory receptors. Instead the CD200R cytoplasmic domain contains a novel phosphotyrosine binding domain, NPXY, which after binding SHIP inhibits ERK, JNK and MAPK p38 pathways. In activated macrophages signaling results in inhibition of TNFalpha secretion. Isoforms of CD200R have been identified and are thought to play a major role in differentiation, especially in regards to tolerogenic DCs. Expression is restricted to hematopoietic cells: myeloid cells (monocytes, macrophages, DCs, neutrophils, mast cells and basophils) and a subset of T lymphocytes as well as langerhans (LC) cells and dendritic epidermal T cells. The epitope of OX108 is thought to be near the binding site of CD200. Applications Reported: This OX108 antibody has been reported for use in flow cytometric analysis. Applications Tested: This OX108 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- OX108
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Identification of a novel conserved signaling motif in CD200 receptor required for its inhibitory function.
Autologous transfusion of "old" red blood cells-induced M2 macrophage polarization through IL-10-Nrf2-HO-1 signaling complexes.
TLR10 Senses HIV-1 Proteins and Significantly Enhances HIV-1 Infection.
Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues.
CD64-directed microtubule associated protein tau kills leukemic blasts ex vivo.
Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer.
Analysis of leukocyte membrane protein interactions using protein microarrays.
Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor.
Timmerman LM, de Graaf JF, Satravelas N, Kesmir Ç, Meyaard L, van der Vlist M
PloS one 2021;16(3):e0244770
PloS one 2021;16(3):e0244770
Autologous transfusion of "old" red blood cells-induced M2 macrophage polarization through IL-10-Nrf2-HO-1 signaling complexes.
Li ZZ, Zhang ZW, Wang H, Chen YQ, Zhou XF, Duan LS, Wang XX, Xu F, Guo JR
Advances in clinical and experimental medicine : official organ Wroclaw Medical University 2020 Jul;29(7):833-840
Advances in clinical and experimental medicine : official organ Wroclaw Medical University 2020 Jul;29(7):833-840
TLR10 Senses HIV-1 Proteins and Significantly Enhances HIV-1 Infection.
Henrick BM, Yao XD, Zahoor MA, Abimiku A, Osawe S, Rosenthal KL
Frontiers in immunology 2019;10:482
Frontiers in immunology 2019;10:482
Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues.
Cella M, Gamini R, Sécca C, Collins PL, Zhao S, Peng V, Robinette ML, Schettini J, Zaitsev K, Gordon W, Bando JK, Yomogida K, Cortez V, Fronick C, Fulton R, Lin LL, Gilfillan S, Flavell RA, Shan L, Artyomov MN, Bowman M, Oltz EM, Jelinsky SA, Colonna M
Nature immunology 2019 Aug;20(8):980-991
Nature immunology 2019 Aug;20(8):980-991
CD64-directed microtubule associated protein tau kills leukemic blasts ex vivo.
Mladenov R, Hristodorov D, Cremer C, Gresch G, Grieger E, Schenke L, Klose D, Amoury M, Woitok M, Jost E, Brümmendorf TH, Fendel R, Fischer R, Stein C, Thepen T, Barth S
Oncotarget 2016 Oct 11;7(41):67166-67174
Oncotarget 2016 Oct 11;7(41):67166-67174
Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer.
Ramsay AG, Clear AJ, Fatah R, Gribben JG
Blood 2012 Aug 16;120(7):1412-21
Blood 2012 Aug 16;120(7):1412-21
Analysis of leukocyte membrane protein interactions using protein microarrays.
Letarte M, Voulgaraki D, Hatherley D, Foster-Cuevas M, Saunders NJ, Barclay AN
BMC biochemistry 2005 Mar 1;6:2
BMC biochemistry 2005 Mar 1;6:2
Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor.
Foster-Cuevas M, Wright GJ, Puklavec MJ, Brown MH, Barclay AN
Journal of virology 2004 Jul;78(14):7667-76
Journal of virology 2004 Jul;78(14):7667-76
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD4 eFluor® 450 (Product # 48-0048-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD200 Receptor PE (purple histogram). Cells in the monocyte (left) or lymphocyte (right) gates were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.pone.0244770.g003 Fig 3 U937-CD200R mutants express CD200R on their surface. A) Conserved residues of CD200R in mammals were mutated as indicated. B) U937 cells were transduced with different CD200R mutants and tested for their CD200R surface expression by flow cytometry. Representative figures of the gating strategy are shown. First the population of live cells was selected. From these live cells, single cells were selected and from these single cells CD200R expressing cells were selected for phosflow analysis. SSC-A: side scatter area; FSC-A: forward scatter area; FSC-W: forward scatter width. Control: staining with the secondary antibody only.
- Conjugate
- Yellow dye