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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 14-9201-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD200 Receptor Monoclonal Antibody (OX108), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody OX108 recognizes human CD200R also known as OX2. CD200R is an inhibitory receptor with a similar structure as its ligand, CD200, yet lacks an ITIM domain typically found in inhibitory receptors. Instead the CD200R cytoplasmic domain contains a novel phosphotyrosine binding domain, NPXY, which after binding SHIP inhibits ERK, JNK and MAPK p38 pathways. In activated macrophages signaling results in inhibition of TNFalpha secretion. Isoforms of CD200R have been identified and are thought to play a major role in differentiation, especially in regards to tolerogenic DCs.
- Antibody clone number
- OX108
- Concentration
- 0.5 mg/mL
Submitted references Identification of a novel conserved signaling motif in CD200 receptor required for its inhibitory function.
Analysis of leukocyte membrane protein interactions using protein microarrays.
Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor.
Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.
Timmerman LM, de Graaf JF, Satravelas N, Kesmir Ç, Meyaard L, van der Vlist M
PloS one 2021;16(3):e0244770
PloS one 2021;16(3):e0244770
Analysis of leukocyte membrane protein interactions using protein microarrays.
Letarte M, Voulgaraki D, Hatherley D, Foster-Cuevas M, Saunders NJ, Barclay AN
BMC biochemistry 2005 Mar 1;6:2
BMC biochemistry 2005 Mar 1;6:2
Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor.
Foster-Cuevas M, Wright GJ, Puklavec MJ, Brown MH, Barclay AN
Journal of virology 2004 Jul;78(14):7667-76
Journal of virology 2004 Jul;78(14):7667-76
Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.
Wright GJ, Cherwinski H, Foster-Cuevas M, Brooke G, Puklavec MJ, Bigler M, Song Y, Jenmalm M, Gorman D, McClanahan T, Liu MR, Brown MH, Sedgwick JD, Phillips JH, Barclay AN
Journal of immunology (Baltimore, Md. : 1950) 2003 Sep 15;171(6):3034-46
Journal of immunology (Baltimore, Md. : 1950) 2003 Sep 15;171(6):3034-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0048-42) and 0.25 µg of Purified Mouse IgG1 kappa Isotype Control (Product # 14-4714-82) (left) or 0.25 µg of Anti-Human CD200 Receptor Purified (right) followed by Anti-Mouse IgG PE.Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.pone.0244770.g003 Fig 3 U937-CD200R mutants express CD200R on their surface. A) Conserved residues of CD200R in mammals were mutated as indicated. B) U937 cells were transduced with different CD200R mutants and tested for their CD200R surface expression by flow cytometry. Representative figures of the gating strategy are shown. First the population of live cells was selected. From these live cells, single cells were selected and from these single cells CD200R expressing cells were selected for phosflow analysis. SSC-A: side scatter area; FSC-A: forward scatter area; FSC-W: forward scatter width. Control: staining with the secondary antibody only.
