AF3414

antibody from R&D Systems

Targeting: CD200R1 CD200R, HCRTR2, MOX2R, OX2R

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF3414

Provider

R&D Systems

Product name

Human CD200R1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human CD200 R1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse CD200 R1 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

AAQ19772


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer.

Ramsay AG, Clear AJ, Fatah R, Gribben JG
Blood 2012 Aug 16;120(7):1412-21

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CD200 R1 in Human Immature Dentritic Cells by Flow Cytometry. Human immature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14⁺ cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 6-7 days were stained with Goat Anti-Human CD200 R1 Antigen Affinity-purified Polyclonal Antibody(Catalog # AF3414, filled histogram) or isotype control (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin (Catalog # F0107).