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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NB100-96920 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-96920, RRID:AB_1291295
- Product name
- Goat Polyclonal Monoglyceride Lipase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_009214.1
- Reactivity
- Human, Mouse
- Host
- Goat
- Antigen sequence
QDLPHLVNADGQY- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol.
Blankman JL, Simon GM, Cravatt BF
Chemistry & biology 2007 Dec;14(12):1347-56
Chemistry & biology 2007 Dec;14(12):1347-56
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Monoglyceride Lipase Antibody [NB100-96920] - Staining of Human Frontal Cortex (A) and Mouse Adipose (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Monoglyceride Lipase Antibody [NB100-96920] - Staining of Mouse Adipose lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NB100-96920] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NB100-96920] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Monoglyceride Lipase Antibody [NB100-96920] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
