PAB9929

antibody from Abnova Corporation

Targeting: AGAP2 CENTG1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB9929

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB9929, RRID:AB_1672231

Product name

AGAP2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against partial recombinant AGAP2.

Storage

Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The Arf GAPs AGAP1 and AGAP2 distinguish between the adaptor protein complexes AP-1 and AP-3.

Nie Z, Fei J, Premont RT, Randazzo PA
Journal of cell science 2005 Aug 1;118(Pt 15):3555-66

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PIKE-A is amplified in human cancers and prevents apoptosis by up-regulating Akt.

Ahn JY, Hu Y, Kroll TG, Allard P, Ye K
Proceedings of the National Academy of Sciences of the United States of America 2004 May 4;101(18):6993-8

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GGAPs, a new family of bifunctional GTP-binding and GTPase-activating proteins.

Xia C, Ma W, Stafford LJ, Liu C, Gong L, Martin JF, Liu M
Molecular and cellular biology 2003 Apr;23(7):2476-88

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using AGAP2 polyclonal antibody (Cat # PAB9929) shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates.No specific band staining is noted in mocktransfected cell lysates (empty vector).The membrane was probed with the primary antibody diluted to 1 : 10,000.Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.