Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP2-27205 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Sirtuin 1/SIRT1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Feline, Hamster, Porcine, Rabbit, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Comparison of the effects of resveratrol and its derivative pterostilbene on hepatic oxidative stress and mitochondrial dysfunction in piglets challenged with diquat.
Effects of IGF-1 isoforms on muscle growth and sarcopenia.
Resveratrol attenuates mitochondrial dysfunction in the liver of intrauterine growth retarded suckling piglets by improving mitochondrial biogenesis and redox status.
Moderate chronic administration of Vineatrol-enriched red wines improves metabolic, oxidative, and inflammatory markers in hamsters fed a high-fat diet.
Zhang H, Chen Y, Chen Y, Jia P, Ji S, Xu J, Li Y, Wang T
Food & function 2020 May 1;11(5):4202-4215
Food & function 2020 May 1;11(5):4202-4215
Effects of IGF-1 isoforms on muscle growth and sarcopenia.
Ascenzi F, Barberi L, Dobrowolny G, Villa Nova Bacurau A, Nicoletti C, Rizzuto E, Rosenthal N, Scicchitano BM, Musarò A
Aging cell 2019 Jun;18(3):e12954
Aging cell 2019 Jun;18(3):e12954
Resveratrol attenuates mitochondrial dysfunction in the liver of intrauterine growth retarded suckling piglets by improving mitochondrial biogenesis and redox status.
Zhang H, Li Y, Su W, Ying Z, Zhou L, Zhang L, Wang T
Molecular nutrition & food research 2017 May;61(5)
Molecular nutrition & food research 2017 May;61(5)
Moderate chronic administration of Vineatrol-enriched red wines improves metabolic, oxidative, and inflammatory markers in hamsters fed a high-fat diet.
Romain C, Bresciani L, Gaillet S, Feillet-Coudray C, Calani L, Bonafos B, Vidé J, Rugani N, Ramos J, Del Rio D, Cristol JP, Rouanet JM
Molecular nutrition & food research 2014 Jun;58(6):1212-25
Molecular nutrition & food research 2014 Jun;58(6):1212-25
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Simple Western lane view shows a specific band for Sirtuin 1/SIRT1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.