Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG54721 - Provider product page
- Provider
- Arigo
- Product name
- anti-SIRT1 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 566-601 (C-terminus) of Mouse Sirt1 protein.
- Description
- Protein A and Immunogen affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of mouse F9 cell line lysate stained with ARG54721 anti-SIRT1 antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: A549 cells stained with ARG54721 anti-SIRT1 antibody at 1:25 dilution. Alexa Fluor 488-conjugated secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Mouse brain section stained with ARG54721 anti-SIRT1 antibody at 1:25 dilution.
