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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 690502 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#690502, RRID:AB_2629822
- Product name
- Purified anti-SIRT1
- Antibody type
- Monoclonal
- Description
- SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuates p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W16003A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from human HeLa cells (lane 1), 293T cells (lane 2), and NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-SIRT1 (clone W16003A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
- Conjugate
- Unconjugated
