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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10318 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10318, RRID:AB_11183260
- Product name
- SIRT1 monoclonal antibody, clone 1F3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant SIRT1.
- Isotype
- IgG
- Antibody clone number
- 1F3
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) against MCF-7 (1), Jurkat (2), HeLa (3), HEK293 (4) and A-549 (5) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NTERA-2 cells using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (A) and kidney cancer tissue (B) using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of K-562 cells using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) (green) and negative control (purple).
- Validation comment
- Flow Cytometry
