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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [2]
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- Product number
- BML-SA427-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-SA427-0100, RRID:AB_2052554
- Product name
- SIRT1 (human) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full length protein
- Description
- Protein A-affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references Obesity depresses the anti-inflammatory HSP70 pathway, contributing to NAFLD progression.
Expression of silent mating type information regulator 2 homolog 1 and its role in human intervertebral disc cell homeostasis.
Di Naso FC, Porto RR, Fillmann HS, Maggioni L, Padoin AV, Ramos RJ, Mottin CC, Bittencourt A, Marroni NA, de Bittencourt PI Jr
Obesity (Silver Spring, Md.) 2015 Jan;23(1):120-9
Obesity (Silver Spring, Md.) 2015 Jan;23(1):120-9
Expression of silent mating type information regulator 2 homolog 1 and its role in human intervertebral disc cell homeostasis.
Zhang Z, Kakutani K, Maeno K, Takada T, Yurube T, Doita M, Kurosaka M, Nishida K
Arthritis research & therapy 2011;13(6):R200
Arthritis research & therapy 2011;13(6):R200
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of recombinant human sirtuins, Jurkat cells and HeLa nuclear extract. The nitrocellulose was probed with anti- SIRT1 at 1:1000. The secondary antibody was coupled to alkaline phosphatase and the blot was developed with BCIP/NBT. Arrows indicate positions of molecular weight markers. Lanes: S2, S3, S5 (SIRTs 2, 3 & 5, 50 ng each); J (150,000 Jurkat cells); H (18 _g HeLa nuclear extract); S1 (SIRT1- 5 ng, 0.5 ng).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of formalin fixed, paraffin-embedded human liver tumor tissue using SIRT1 antibody (Prod. no. BML-SA427) showing nuclear staining of hepatocytes.
