Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00010226-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010226-M01, RRID:AB_1112365
- Product name
- PLIN3 monoclonal antibody (M01), clone 2F7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant PLIN3.
- Antigen sequence
MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLI
SSTCDMVSAAYASTKESYPHVKTVCDAAEKGVRTL
TAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEE
NLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAK
DTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQS
VMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAE
LARIATSLDGFDVASVQQQRQEQSYFVRLGSLSER
LRQHAYEHSLGKLRATKQRAQEALLQLSQALSLME
TVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKE
PPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQG
LPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSS
ILAQSRERVASAREALDHMVEYVAQNTPVTWLVGP
FAPGITEKAPEEKK- Isotype
- IgG
- Antibody clone number
- 2F7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PLIN3 expression in transfected 293T cell line by PLIN3 monoclonal antibody (M01), clone 2F7.Lane 1: PLIN3 transfected lysate (Predicted MW: 47 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PLIN3 is 3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PLIN3 transfected lysate using anti-PLIN3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PLIN3 monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to PLIN3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol