H00010226-M01

antibody from Abnova Corporation

Targeting: PLIN3 M6PRBP1, PP17, TIP47

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010226-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010226-M01, RRID:AB_1112365

Product name

PLIN3 monoclonal antibody (M01), clone 2F7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant PLIN3.

Antigen sequence

MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLI
SSTCDMVSAAYASTKESYPHVKTVCDAAEKGVRTL
TAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEE
NLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAK
DTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQS
VMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAE
LARIATSLDGFDVASVQQQRQEQSYFVRLGSLSER
LRQHAYEHSLGKLRATKQRAQEALLQLSQALSLME
TVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKE
PPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQG
LPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSS
ILAQSRERVASAREALDHMVEYVAQNTPVTWLVGP
FAPGITEKAPEEKK


Isotype

IgG

Antibody clone number

2F7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PLIN3 expression in transfected 293T cell line by PLIN3 monoclonal antibody (M01), clone 2F7.Lane 1: PLIN3 transfected lysate (Predicted MW: 47 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PLIN3 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of PLIN3 transfected lysate using anti-PLIN3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PLIN3 monoclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PLIN3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol