10694-1-AP
antibody from Invitrogen Antibodies
Targeting: PLIN3
M6PRBP1, PP17, TIP47
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 10694-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIP47 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: EMVSSAKDT VATQLSEAVD ATRGAVQSGV DKTKSVVTGG VQSVMGSRLG QMVLSGVDTV LGKSEEWADN HLPLTDAELA RIATSLDGFD VASVQQQRQE QSYFVRLGSL SERLRQHAYE HSLGKLRATK QRAQEALLQL SQALSLMETV KQGVDQKLVE GQEKLHQMWL SWNQKQLQGP EKEPPKPEQV ESRALTMFRD IAQQLQATCT SLGSSIQGLP TNVKDQVQQA RRQVEDLQAT FSSIHSFQDL SSSILAQSRE RVASAREALD HMVEYVAQNT PVTWLVGPFA PGITEKAPEE KK (134-434 aa encoded by BC007566)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of anti-TIP47 (10694-1-AP,1:500) with MEF cell by Dr.Hector Alex Saka.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed oleic acid treated HeLa cells using 10694-1-AP (TIP47 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 10694-1-AP (TIP47 antibody) at dilution of 1:50 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 10694-1-AP (TIP47 antibody) at dilution of 1:50 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 10694-1-AP ( TIP47 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 10694-1-AP ( TIP47 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug TIP47 antibody (10694-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TIP47 (IP:10694-1-AP, 3ug; Detection:10694-1-AP 1:1000) with HeLa cells lysate 1000ug.