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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-80810 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- METTL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human METTL1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of METTL1 in Lane A: Jurkat Whole Cell Lysate (30 µg), Lane B: 293T Whole Cell Lysate (30 µg) . Samples were probed using a METTL1 Polyclonal Antibody (Product # PA5-80810) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:31 kDa. Observed band size:34 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using METTL1 Polyclonal Antibody (Product # PA5-80810) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human METTL1 in human bladder carcinoma with METTL1 Polyclonal Antibody (Product # PA5-80810, 1:5,000 dilution, formalin-fixed paraffin embedded sections). Left panel: tissue incubated with primary antibody. Right panel: tissue incubated with mixture of primary antibody and antigen (recombinant protein).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- METTL1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate 0.5 µL with METTL1 Polyclonal Antibody (Product # PA5-80810) and 15 µL of 50 % Protein G agarose. Primary antibody: METTL1 Polyclonal Antibody, at 1:1,000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.
