14994-1-AP

antibody from Invitrogen Antibodies

Targeting: METTL1 C12orf1, TRM8, TRMT8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14994-1-AP

Provider

Invitrogen Antibodies

Product name

METTL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAAETRNVA GAEAPPPQKR YYRQRAHSNP MADHTLRYPV KPEEMDWSEL YPEFFAPLTQ NQSHDDPKDK KEKRAQAQVE FADIGCGYGG LLVELSPLFP DTLILGLEIR VKVSDYVQDR IRALRAAPAG GFQNIACLRS NAMKHLPNFF YKGQLTKMFF LFPDPHFKRT KHKWRIISPT LLAEYAYVLR VGGLVYTITD VLELHDWMCT HFEEHPLFER VPLEDLSEDP VVGHLGTSTE EGKKVLRNGG KNFPAIFRRI QDPVLQAVTS QTSLPGH (1-276 aa encoded by BC000550)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A2780 cells were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 14994-1-AP (METTL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 14994-1-AP (METTL1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 14994-1-AP (METTL1 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-METTL1 (IP:14994-1-AP, 4ug; Detection:14994-1-AP 1:300) with A2780 cells lysate 3600 ug.