MA5-41071

antibody from Invitrogen Antibodies

Targeting: METTL1 C12orf1, TRM8, TRMT8

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-41071

Provider

Invitrogen Antibodies

Product name

METTL1 Monoclonal Antibody (9C2F2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

9C2F2

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of METTL1 in Lane A: 293T Whole Cell lysate, Lane B: Jurkat Whole Cell Lysate. Samples (30 µg per lane) were incubated with monoclonal antibody (Product # MA5-41071) with a dilution of 1:500 , followed by Goat Anti-Mouse IgG H&L (Dylight 800) using a dilution of 1:15,000. Assay was performed under reducing conditions. Predicted band size: 31 kDa, Observed band size: 31 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using METTL1 Monoclonal Antibody (9C2F2) (Product # MA5-41071) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 31 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

METTL1 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate, Lane B: 0.5 mg Jurkat Whole Cell Lysate 2 µL with METTL1 Monoclonal Antibody (9C2F2) (Product # MA5-41071) and 15 µL of 50 % Protein G agarose. Primary antibody: METTL1 Monoclonal Antibody (9C2F2), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7,500 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.