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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX101682 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101682, RRID:AB_1949598
- Product name
- GRK2 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Astragaloside IV alleviates E. coli-caused peritonitis via upregulation of neutrophil influx to the site of infection.
Crosstalk between adrenergic and toll-like receptors in human mesenchymal stem cells and keratinocytes: a recipe for impaired wound healing.
Huang P, Lu X, Yuan B, Liu T, Dai L, Liu Y, Yin H
International immunopharmacology 2016 Oct;39:377-382
International immunopharmacology 2016 Oct;39:377-382
Crosstalk between adrenergic and toll-like receptors in human mesenchymal stem cells and keratinocytes: a recipe for impaired wound healing.
Dasu MR, Ramirez SR, La TD, Gorouhi F, Nguyen C, Lin BR, Mashburn C, Stewart H, Peavy TR, Nolta JA, Isseroff RR
Stem cells translational medicine 2014 Jun;3(6):745-59
Stem cells translational medicine 2014 Jun;3(6):745-59
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A:NIH-3T37.5% SDS PAGE GTX101682 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g whole cell lysate)A:MOLT4 (GTX27912)7.5% SDS PAGEGTX101682 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GRK2 antibody detects GRK2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX101682) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GRK2(GTX101682) antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 £gg of GRK2 antibody [C2C3], C-term (GTX101682).Western blot analysis was performed using GRK2 antibody [C2C3], C-term (GTX101682).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GRK2(GTX101682) antibody at 1:500 dilution.
