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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA5-15840 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRK2 Monoclonal Antibody (3F8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15840 targets GRK2 in IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
- Antibody clone number
- 3F8
- Concentration
- Conc. Not Determined
Submitted references GRK2 mediates β-arrestin interactions with 5-HT(2) receptors for JC polyomavirus endocytosis.
A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer.
Mayberry CL, Wilczek MP, Fong TM, Nichols SL, Maginnis MS
Journal of virology 2021 Mar 10;95(7)
Journal of virology 2021 Mar 10;95(7)
A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer.
Buchholz M, Honstein T, Kirchhoff S, Kreider R, Schmidt H, Sipos B, Gress TM
PloS one 2015;10(4):e0122946
PloS one 2015;10(4):e0122946
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GRK2 using GRK2 monoclonal antibody (Product # MA5-15840) in HeLa (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), COS-7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GRK2 using GRK2 monoclonal antibody (Product # MA5-15840) in HeLa (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), COS-7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of GRK2 was achieved by transfecting HEK-293 cells with GRK2 specific siRNAs (Silencer® select Product # s1129). Western blot analysis (Fig. a) was performed using whole cell extracts from the GRK2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GRK2 Monoclonal Antibody (Product # MA5-15840, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GRK2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of RAW 264.7 (Lane 1), THP-1 (Lane 2), HEK-293 (Lane 3), HEK-293 treated with Nocodazole (5uM, 24 hrs) (Lane 4), Raji (Lane 5), MOLT-4 (Lane 6) and NIH-3T3 (Lane 7). The blot was probed with GRK2 Monoclonal Antibody (3F8) (Product # MA5-15840, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). A 79 kDa band corresponding to GRK2 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using GRK2 monoclonal antibody (Product # MA5-15840) (Green). Blue: DRAQ5 fluorescent DNA dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GRK2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GRK2 Monoclonal Antibody (3F8) (Product # MA5-15840) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 monoclonal antibody (Product # MA5-15840) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 5 ADRBK1 overexpression promotes growth in human PDAC. A: Box-and-whisker plot showing ADRBK1 mRNA expression in primary human pancreatic tumor tissue samples, chronic pancreatitis and normal pancreas as analyzed by quantitative realtime reverse transcription PCR (qRT-PCR). Expression was normalized to ribosomal protein, large, P0 (RPLP0) mRNA levels. Data in Fig. represent median and 2nd and 3rd quartiles (boxes) as well as minimum and maximum values (whiskers). CP = Chronic Pancreatitis. ** p
