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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C101194 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C101194, RRID:AB_2299260
- Product name
- PCK1 Antibody (aa592-622) LS-C101194
- Antibody type
- Polyclonal
- Description
- Protein A purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of PCK1 Antibody in rat primary hepatocyte cell line lysates. PCK1 (arrow) was detected using the purified antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PCK1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The PCK1 antibody detected the PCK1 protein (arrow).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- The anti-PCK1 antibody is used in Western blot to detect PCK1 in mouse liver (Lane 1) and mouse small intestine tissue lysates (Lane 2).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
