TA590679

antibody from OriGene

Targeting: PCK1 PEPCK-C

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA590679

Provider

OriGene

Product name

Rabbit Polyclonal PCK1 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal PCK1 Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

PCK1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.05mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot: PCK1 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 69 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCK1 (RC204758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCK1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PCK1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

ELISA: PCK1 Antibody

Validation comment

ELISA

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PCK1 Rabbit Polyclonal antibody. (TA590679)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PCK1 Rabbit Polyclonal antibody. (TA590679)

Validation comment

IHC