16754-1-AP

antibody from Invitrogen Antibodies

Targeting: PCK1 PEPCK-C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16754-1-AP

Provider

Invitrogen Antibodies

Product name

PCK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EGEKKYLAA AFPSACGKTN LAMMNPSLPG WKVECVGDDI AWMKFDAQGH LRAINPENGF FGVAPGTSVK TNPNAIKTIQ KNTIFTNVAE TSDGGVYWEG IDEPLASGVT ITSWKNKEWS SEDGEPCAHP NSRFCTPASQ CPIIDAAWES PEGVPIEGII FGGRRPAGVP LVYEALSWQH GVFVGAAMRS EATAAAEHKG KIIMHDPFAM RPFFGYNFGK YLAHWLSMAQ HPAAKLPKIF HVNWFRKDKE GKFLWPGFGE NSRVLEWMFN RIDGKASTKL TPIGYIPKED ALNLKGLGHI NMMELFSISK EFWEKEVEDI EKYLEDQVNA DLPCEIEREI LALKQRISQM (274-622 aa encoded by BC023978)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16754-1-AP (PCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16754-1-AP (PCK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16754-1-AP (PCK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat liver tissue were subjected to SDS PAGE followed by western blot with 16754-1-AP (PCK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 16754-1-AP (PCK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16754-1-AP ( PCK1 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16754-1-AP ( PCK1 Antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PCK1 (IP:16754-1-AP, 4ug; Detection:16754-1-AP 1:500) with HepG2 cells lysate 3600ug.