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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF7316 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat PCK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, rat PCK1 in Western blots, and detects recombinant human PCK1 in direct ELISAs. In direct ELISAs, less than 3% cross-reactivity with recombinant mouse PCK1 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P35558- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat PCK1 by Western Blot. Western blot shows lysates of human liver tissue, human kidney tissue, mouse kidney tissue, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PCK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7316) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PCK1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
