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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00005511-M05 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005511-M05, RRID:AB_1199937
- Product name
- Mouse Monoclonal NIPP1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PPP1R8 - protein phosphatase 1, regulatory (inhibitor) subunit 8 (4B5)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NIPP1 Antibody (4B5) [H00005511-M05] - Analysis of PPP1R8 expression in transfected 293T cell line by PPP1R8 monoclonal antibody (M05), clone 4B5. Lane 1: PPP1R8 transfected lysate (Predicted MW: 38.5 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: NIPP1 Antibody (4B5) [H00005511-M05] - Detection limit for recombinant GST tagged PPP1R8 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NIPP1 Antibody (4B5) [H00005511-M05] - Analysis of PPP1R8 transfected lysate using anti-PPP1R8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPP1R8 monoclonal antibody.
