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LearnPA5-121027
antibody from Invitrogen Antibodies
Targeting: PPP1R8
ard-1, ARD1, NIPP-1, NIPP1, PRO2047
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-121027 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP1R8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: HL-60, PC-12, HeLa, HepG2, 293T, Mouse brain. The target is usually found in the following locations: Cytoplasm, Nucleus, Nucleus speckle.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 4.73 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PPP1R8 in various cell lines using a PPP1R8 Polyclonal antibody (Product # PA5-121027) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PPP1R8 in various cell lines using a PPP1R8 Polyclonal antibody (Product # PA5-121027) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of PPP1R8 in MCF7 cells using a PPP1R8 Polyclonal antibody (Product # PA5-121027).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PPP1R8 in human breast cancer using a Polyclonal antibody (Product # PA5-121027) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PPP1R8 in human breast cancer using a Polyclonal antibody (Product # PA5-121027) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PPP1R8 in human breast cancer using a Polyclonal antibody (Product # PA5-121027) at a dilution of 1:100.
