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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-1286 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1286, RRID:AB_2268695
- Product name
- Goat Polyclonal NIPP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise all three human isoforms of this protein.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing.
The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly.
Nuytten M, Beke L, Van Eynde A, Ceulemans H, Beullens M, Van Hummelen P, Fuks F, Bollen M
Oncogene 2008 Feb 28;27(10):1449-60
Oncogene 2008 Feb 28;27(10):1449-60
The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly.
Beullens M, Bollen M
The Journal of biological chemistry 2002 May 31;277(22):19855-60
The Journal of biological chemistry 2002 May 31;277(22):19855-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NIPP1 Antibody [NB100-1286] - (1ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and NIH3T3 (D) nuclear lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286] - 3.8ug/ml staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
