18047-1-AP

antibody from Invitrogen Antibodies

Targeting: CASC3 BTZ, MLN51

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18047-1-AP

Provider

Invitrogen Antibodies

Product name

MLN51/CASC3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LEQTSVRDP SPEADAPVLG SPEKEEAASE PPAAAPDAAP PPPDRPIEKK SYSRARRTRT KVGDAVKLAE EVPPPPEGLI PAPPVPETTP TPPTKTGTWE APVDSSTSGL EQDVAQLNIA EQNWSPGQPS FLQPRELRGM PNHIHMGAGP PPQFNRMEEM GVQGGRAKRY SSQRQRPVPE PPAPPVHISI MEGHYYDPLQ FQGPIYTHGD SPAPLPPQGM LVQPGMNLPH PGLHPHQTPA PLPNPGLYPP PVSMSPGQPP PQQLLAPTYF SAPGVMNFGN PSYPYAPGAL PPPPPPHLYP NTQAPSQVYG GVTYYNPAQQ QVQPKPSPPR RTPQPVTIKP PPPEVVSRGS S (354-703 aa encoded by BC050526)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A2780 cells were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SKOV-3 cells were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CASC3 antibody 18047-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CASC3 antibody 18047-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).