MA5-25494

antibody from Invitrogen Antibodies

Targeting: HAND1 bHLHa27, eHand, Hxt, Thing1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA5-25494

Provider

Invitrogen Antibodies

Product name

HAND1 Monoclonal Antibody (OTI1G10)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1G10

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HAND1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with HAND1 (Product # MA5-25494) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HAND1 in human tissue (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon) samples using 10 µg per lane. Samples were separated by SDS-PAGE and probed with HAND1 (Product # MA5-25494) monoclonal antibody at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HAND1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with HAND1 (Product # MA5-25494) monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HAND1 in COS7 cells. Cells were transfected with a plasmid overexpressing HAND1 and probed with a HAND1 monoclonal antibody (Product # MA5-25494).