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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX108979 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108979, RRID:AB_10618065
- Product name
- CDC6 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references The protein phosphatase 2A regulatory subunit PR70 is a gonosomal melanoma tumor suppressor gene.
van Kempen LC, Redpath M, Elchebly M, Klein KO, Papadakis AI, Wilmott JS, Scolyer RA, Edqvist PH, Pontén F, Schadendorf D, van Rijk AF, Michiels S, Dumay A, Helbling-Leclerc A, Dessen P, Wouters J, Stass M, Greenwood CM, Ghanem GE, van den Oord J, Feunteun J, Spatz A
Science translational medicine 2016 Dec 14;8(369):369ra177
Science translational medicine 2016 Dec 14;8(369):369ra177
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cdc6 antibody detects CDC6 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract C. 30 ?g C8D30 whole cell lysate/extract D. 30 ?g BCL-1 whole cell lysate/extract E. 30 ?g Raw264.7 whole cell lysate/extract F. 30 ?g C2C12 whole cell lysate/extract7.5% SDS-PAGECdc6 antibody (GTX108979) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cdc6 antibody detects CDC6 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract B. 30 ?g Rat2 whole cell lysate/extract7.5% SDS-PAGECdc6 antibody (GTX108979) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: JurKat 7.5% SDS PAGE GTX108979 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CDC6 antibody (GTX108979) diluted at 1:500 and competitor's antibody (sc-8341) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC6 antibody (GTX108979) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CDC6 antibody (GTX108979) diluted at 1:500 and competitor's antibody (sc-8341) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cdc6(GTX108979) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDC6 antibody detects CDC6 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDC6 protein stained by CDC6 antibody (GTX108979) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDC6 antibody detects CDC6 protein at cytoplasm in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. CDC6 antibody (GTX108979) diluted at 1:500.
