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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PAB0435 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0435, RRID:AB_982431
- Product name
- CDC6 (phospho S54) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC6.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cdc6 chromatin affinity is unaffected by serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A.
Caspase-3-mediated cleavage of Cdc6 induces nuclear localization of p49-truncated Cdc6 and apoptosis.
Human replication protein Cdc6 prevents mitosis through a checkpoint mechanism that implicates Chk1.
Alexandrow MG, Hamlin JL
Molecular and cellular biology 2004 Feb;24(4):1614-27
Molecular and cellular biology 2004 Feb;24(4):1614-27
Caspase-3-mediated cleavage of Cdc6 induces nuclear localization of p49-truncated Cdc6 and apoptosis.
Yim H, Jin YH, Park BD, Choi HJ, Lee SK
Molecular biology of the cell 2003 Oct;14(10):4250-9
Molecular biology of the cell 2003 Oct;14(10):4250-9
Human replication protein Cdc6 prevents mitosis through a checkpoint mechanism that implicates Chk1.
Clay-Farrace L, Pelizon C, Santamaria D, Pines J, Laskey RA
The EMBO journal 2003 Feb 3;22(3):704-12
The EMBO journal 2003 Feb 3;22(3):704-12
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The CDC6 (phospho S54) polyclonal antibody (Cat # PAB0435) is used in Western blot to detect Phospho-CDC6-S54 in Ramos cell lysate
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDC6 (phospho S54) polyclonal antibody (Cat # PAB0435). Western blot analysis of CDC6 expression in HeLa.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDC6 (phospho S54) polyclonal antibody (Cat # PAB0435). Western blot analysis of CDC6 expression in human colon.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC6 (phospho S54) polyclonal antibody (Cat # PAB0435) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
