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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- MA5-14314 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cdc6 Monoclonal Antibody (DCS-180)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-14314 targets CDC6 in IP and WB applications and shows reactivity with Human samples.
- Antibody clone number
- DCS-180
- Concentration
- 0.2 mg/mL
Submitted references The abundance of Rad51 protein in mouse embryonic stem cells is regulated at multiple levels.
Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 protein undergoes C-terminal phosphorylation.
Inactivation and disassembly of the anaphase-promoting complex during human cytomegalovirus infection is associated with degradation of the APC5 and APC4 subunits and does not require UL97-mediated phosphorylation of Cdh1.
First Cdc7 kinase inhibitors: pyrrolopyridinones as potent and orally active antitumor agents. 2. Lead discovery.
RNA-dependent recruitment of the origin recognition complex.
Cell cycle regulation of the human Six1 homeoprotein is mediated by APC(Cdh1).
Tichy ED, Pillai R, Deng L, Tischfield JA, Hexley P, Babcock GF, Stambrook PJ
Stem cell research 2012 Sep;9(2):124-34
Stem cell research 2012 Sep;9(2):124-34
Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 protein undergoes C-terminal phosphorylation.
Uranbileg B, Yamamoto H, Park JH, Mohanty AR, Arakawa-Takeuchi S, Jinno S, Okayama H
The Journal of biological chemistry 2012 Feb 24;287(9):6275-83
The Journal of biological chemistry 2012 Feb 24;287(9):6275-83
Inactivation and disassembly of the anaphase-promoting complex during human cytomegalovirus infection is associated with degradation of the APC5 and APC4 subunits and does not require UL97-mediated phosphorylation of Cdh1.
Tran K, Kamil JP, Coen DM, Spector DH
Journal of virology 2010 Oct;84(20):10832-43
Journal of virology 2010 Oct;84(20):10832-43
First Cdc7 kinase inhibitors: pyrrolopyridinones as potent and orally active antitumor agents. 2. Lead discovery.
Menichincheri M, Bargiotti A, Berthelsen J, Bertrand JA, Bossi R, Ciavolella A, Cirla A, Cristiani C, Croci V, D'Alessio R, Fasolini M, Fiorentini F, Forte B, Isacchi A, Martina K, Molinari A, Montagnoli A, Orsini P, Orzi F, Pesenti E, Pezzetta D, Pillan A, Poggesi I, Roletto F, Scolaro A, Tatò M, Tibolla M, Valsasina B, Varasi M, Volpi D, Santocanale C, Vanotti E
Journal of medicinal chemistry 2009 Jan 22;52(2):293-307
Journal of medicinal chemistry 2009 Jan 22;52(2):293-307
RNA-dependent recruitment of the origin recognition complex.
Norseen J, Thomae A, Sridharan V, Aiyar A, Schepers A, Lieberman PM
The EMBO journal 2008 Nov 19;27(22):3024-35
The EMBO journal 2008 Nov 19;27(22):3024-35
Cell cycle regulation of the human Six1 homeoprotein is mediated by APC(Cdh1).
Christensen KL, Brennan JD, Aldridge CS, Ford HL
Oncogene 2007 May 17;26(23):3406-14
Oncogene 2007 May 17;26(23):3406-14
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of CDC6 using CDC6 Monoclonal Antibody (Product # MA5-14314) on MDA231 Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Cdc6 was achieved by transfecting HeLa cells with Cdc6 specific siRNAs (Silencer® select Product # s2744 and s2746). Western blot analysis (Fig a) was performed using Modified whole cell extract (1% SDS) from the Cdc6 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-Cdc6 Mouse Monoclonal Antibody (Product # MA5-14314, 0.2 µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to Cdc6.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), HL-60 (Lane 2) and HepG2 (Lane 3). The blots were probed with Anti-CDC6 Monoclonal Antibody (Product # 13-7000, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 62 kDa corresponding to CDC6 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of CDC6 using CDC6 Monoclonal Antibody (Product # MA5-14314) on Native Human Raji Cells.
