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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX60338 - Provider product page
- Provider
- GeneTex
- Product name
- Retinoic Acid Receptor alpha antibody - ChIP grade
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- WB analysis of HEK293T transfected with a RARA construct (lane 2) or with a negative control construct (lane 1) using RAR alpha antibody diluted 1:750 in BSA/PBS-Tween.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of the RAR alpha antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,400.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with NB4 nuclear extract and either 4 £gl of RARA antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to OGG1, FUT7, NFE2, VEGF or H2B.
