18500-1-AP

antibody from Invitrogen Antibodies

Targeting: IGFBP4 BP-4, HT29-IGFBP, IBP4, IGFBP-4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18500-1-AP

Provider

Invitrogen Antibodies

Product name

IGFBP4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MLPLCLVAA LLLAAGPGPS LGDEAIHCPP CSEEKLARCR PPVGCEELVR EPGCGCCATC ALGLGMPCGV YTPRCGSGLR CYPPRGVEKP LHTLMHGQGV CMELAEIEAI QESLQPSDKD EGDHPNNSFS PCSAHDRRCL QKHFAKIRDR STSGGKMKVN GAPREDARPV PQGSCQSELH RALERLAASQ SRTHEDLYII PIPNCDRNGN FHPKQCHPAL DGQRGKCWCV DRKTGVKLPG GLEPKGELDC HQLADSFRE (1-258 aa encoded by BC016041 )

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP ( IGFBP4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of C6 cells using 18500-1-AP (IGFBP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 18500-1-AP ( IGFBP4 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 18500-1-AP ( IGFBP4 Antibody) at dilution of 1:200 (under 40x lens).