AF804

antibody from R&D Systems

Targeting: IGFBP4 BP-4, HT29-IGFBP, IBP4, IGFBP-4

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF804

Provider

R&D Systems

Product name

Human IGFBP-4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human IGFBP-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGFBP-4 is observed, and less than 1% cross-reactivity with recombinant human (rh) IGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-5 and rhIGFBP-6 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

AAA62670


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

EWS-FLI-1 creates a cell surface microenvironment conducive to IGF signaling by inducing pappalysin-1.

Jayabal P, Houghton PJ, Shiio Y
Genes & cancer 2017 Nov;8(11-12):762-770

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Fetal baboon sex-specific outcomes in adipocyte differentiation at 0.9 gestation in response to moderate maternal nutrient reduction.

Tchoukalova YD, Krishnapuram R, White UA, Burk D, Fang X, Nijland MJ, Nathanielsz PW
International journal of obesity (2005) 2014 Feb;38(2):224-30

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IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.

Crosley EJ, Dunk CE, Beristain AG, Christians JK
Reproductive biology and endocrinology : RB&E 2014 Dec 4;12:123

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The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.

McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology 2006 May;130(6):1754-63

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IGFBP-4 in Human Placenta. IGFBP-4 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 5 µg/mL Goat Anti-Human IGFBP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF804) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IGFBP-4 Inhibition of IGF-II-dependent Cell Proliferation and Neutralization by Human IGFBP-4 Antibody. Recombinant Human IGFBP-4 (Catalog # 804-GB) inhibits Recombinant Human IGF-II (Catalog # 292-G2) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP-4 (0.3 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGFBP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF804). The ND₅₀ is typically 12-60 µg/mL.