Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- BAF804 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IGFBP-4 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IGFBP-4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) IGFBP-1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
AAA62670
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
Wunder DM, Mueller MD, Birkhäuser MH, Bersinger NA
Journal of assisted reproduction and genetics 2005 Jun;22(6):257-64
Journal of assisted reproduction and genetics 2005 Jun;22(6):257-64
Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
Wunder DM, Mueller MD, Birkhäuser MH, Bersinger NA
Journal of assisted reproduction and genetics 2005 Jun;22(6):257-64
Journal of assisted reproduction and genetics 2005 Jun;22(6):257-64
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IGFBP-4 in Human Stomach. IGFBP-4 was detected in immersion fixed paraffin-embedded sections of human stomach array using Goat Anti-Human IGFBP-4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF804) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.