Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-24311 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Tensin 4 Monoclonal Antibody (684524)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 684524
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Tensin 4/CTEN at approximately 74 kDa (as indicated) using 10 µg/mL of mouse Anti-human Tensin 4/CTEN Monoclonal Antibody (Product # MA5-24311). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Tensin 4/CTEN at approximately 74 kDa (as indicated) using 10 µg/mL of mouse Anti-human Tensin 4/CTEN Monoclonal Antibody (Product # MA5-24311). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tensin 4 in lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. Samples were incubated with Tensin 4 monoclonal antibody (Product # MA5-24311) using a dilution of 1 µg/mL followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Tensin 4/CTEN at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tensin 4 in lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. Samples were incubated with Tensin 4 monoclonal antibody (Product # MA5-24311) using a dilution of 1 µg/mL followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Tensin 4/CTEN at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tensin 4 in 0.2 mg/mL lysates of HeLa human cervical epithelial carcinoma cell line. Samples were incubated in Tensin 4 monoclonal antibody (Product # MA5-24311) using a dilution of 10 µg/mL. A specific band was detected for Tensin 4/CTEN at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Tensin 4 in immersion fixed paraffin-embedded sections of human prostate. Samples were incubated in Tensin 4 monoclonal antibody (Product # MA5-24311) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells.