Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX109833 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109833, RRID:AB_1950258
- Product name
- Fatty Acid Synthase antibody [N1], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references A novel small molecule liver X receptor transcriptional regulator, nagilactone B, suppresses atherosclerosis in apoE-deficient mice.
Gluconeogenesis, lipogenesis, and HBV replication are commonly regulated by PGC-1α-dependent pathway.
Gui Y, Yao S, Yan H, Hu L, Yu C, Gao F, Xi C, Li H, Ye Y, Wang Y
Cardiovascular research 2016 Oct;112(1):502-14
Cardiovascular research 2016 Oct;112(1):502-14
Gluconeogenesis, lipogenesis, and HBV replication are commonly regulated by PGC-1α-dependent pathway.
Jhuang HJ, Hsu WH, Lin KT, Hsu SL, Wang FS, Chou CK, Lee KH, Tsou AP, Lai JM, Yeh SF, Huang CY
Oncotarget 2015 Apr 10;6(10):7788-803
Oncotarget 2015 Apr 10;6(10):7788-803
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 5% SDS PAGE GTX109833 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate)A: Molt-4 (GTX27912)7.5% SDS PAGEGTX109833 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fatty Acid Synthase antibody [N1], N-term detects FASN protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extractB. 30 ?g Rat2 whole cell lysate/extract5% SDS-PAGEFatty Acid Synthase antibody [N1], N-term (GTX109833) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fatty Acid Synthase(GTX109833) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FASN antibody immunoprecipitates FASN protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 £gg whole cell lysate/extract of FASN protein expressing HeLa cells B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of FASN by 2.5 £gg of FASN antibody (GTX109833) 5% SDS-PAGE The immunoprecipitated FASN protein was detected by FASN antibody (GTX109833) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovarian carainoma, using Fatty Acid Synthase(GTX109833) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
