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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX110470 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110470, RRID:AB_1950257
- Product name
- Fatty Acid Synthase antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:3000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse liver 5% SDS PAGE GTX110470 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 5% SDS PAGE GTX110470 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody (GTX110470) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:3000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fatty Acid Synthase(GTX110470) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Fatty Acid Synthase protein from HeLa whole cell extracts using 5 £gg of Fatty Acid Synthase antibody [N1N2], N-term (GTX110470).Western blot analysis was performed using Fatty Acid Synthase antibody [N1N2], N-term (GTX110470).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OECM1 xenograft, using Fatty Acid Synthase(GTX110470) antibody at 1:500 dilution.
