PAB22407

antibody from Abnova Corporation

Targeting: TJAP1 PILT, TJP4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22407

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22407, RRID:AB_10984000

Product name

TJAP1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant TJAP1.

Antigen sequence

RLDCNLAVQLLKCNKSHFRNHKFADLPCELQDMVR
KHLHSGQEAASPGPAPSLAPGAVVPTSVIARVLEK
PESLLLNSAQS


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with TJAP1 polyclonal antibody (Cat # PAB22407) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with TJAP1 polyclonal antibody (Cat # PAB22407) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and golgi apparatus.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta with TJAP1 polyclonal antibody (Cat # PAB22407) shows strong cytoplasmic positivity in trophoblastic cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)