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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- 12571-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PBX3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: QLMRLDNML LAEGVSGPEK GGGSAAAAAA AAASGGSSDN SIEHSDYRAK LTQIRQIYHT ELEKYEQACN EFTTHVMNLL REQSRTRPIS PKEIERMVGI IHRKFSSIQM QLKQSTCEAV MILRSRFLDA RRKRRNFSKQ ATEILNEYFY SHLSNPYPSE EAKEELAKKC SITVSQSLVK DPKERGSKGS DIQPTSVVSN WFGNKRIRYK KNIGKFQEEA NLYAAKTAVT AAHAVAAAVQ NNQTNSPTTP NSGSSGSFNL PNSGDMFMNM QSLNGDSYQG SQVGANVQSQ VDTLRHVINQ TGGYSDGLGG NSLYSPHNLN ANGGWQDATT PSSVTSPTEG PGSVHSDTSN (19-367 aa encoded by BC016977)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human lung tissue were subjected to SDS PAGE followed by western blot with 12571-1-AP (PBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 12571-1-AP (PBX3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 12571-1-AP (PBX3 antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PBX3 (IP:12571-1-AP, 4ug; Detection:12571-1-AP 1:500) with HL-60 cells lysate 3040ug.
