Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
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Validation data
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- Product number
- LS-C340521 - Provider product page
- Provider
- LSBio
- Product name
- ZNF449 Antibody (clone OTI4C6) LS-C340521
- Antibody type
- Monoclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI4C6
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF449 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF449 (1:2000).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF449 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF449 (1:2000).
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZNF449 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-ZNF449 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZNF449 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-ZNF449 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Human bladder tissue using anti-ZNF449 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZNF449 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-ZNF449 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150).