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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- AM06655SU-N - Provider product page
- Provider
- OriGene
- Product name
- ITK mouse monoclonal antibody, clone 5G6, Ascites
- Antibody type
- Monoclonal
- Description
- ITK mouse monoclonal antibody, clone 5G6, Ascites
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ITK
- Isotype
- IgG
- Antibody clone number
- 5G6
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using ITK mAb against human ITK (AA: 2-120) recombinant protein. (Expected MW is 39.7 kDa)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using ITK mouse mAb against Jurkat cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).
- Validation comment
- FC
