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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
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- Product number
- 14-9752-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-ITK Monoclonal Antibody (2F12), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 2F12 recognizes human, mouse, and rat ITK. ITK (also known as Interleukin-2 inducible T-cell kinase, T-cell-specific kinase, Tyrosine-protein kinase Lyk, Emt, and Tsk) is a member of the Tec family of kinases that include Tec, Btk, Bmx, and Txk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. ITK is highly expressed by T cells and is required for optimal TCR-induced PLCgamma1 activation. Mice congenitally lacking ITK show profound defects in T cell development and signaling. Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1). ITK also plays a role in the development of various T cell mediated inflammatory disorders such as atopic dermatitis, psoriasis, and allergic asthma. Applications Reported: This 2F12 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy. Applications Tested: This 2F12 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2F12
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Regulation of T-cell responses and disease by tec kinase Itk.
Girls homozygous for an IL-2-inducible T cell kinase mutation that leads to protein deficiency develop fatal EBV-associated lymphoproliferation.
The Tec family tyrosine kinases Itk and Rlk regulate the development of conventional CD8+ T cells.
August A, Ragin MJ
International reviews of immunology 2012 Apr;31(2):155-65
International reviews of immunology 2012 Apr;31(2):155-65
Girls homozygous for an IL-2-inducible T cell kinase mutation that leads to protein deficiency develop fatal EBV-associated lymphoproliferation.
Huck K, Feyen O, Niehues T, Rüschendorf F, Hübner N, Laws HJ, Telieps T, Knapp S, Wacker HH, Meindl A, Jumaa H, Borkhardt A
The Journal of clinical investigation 2009 May;119(5):1350-8
The Journal of clinical investigation 2009 May;119(5):1350-8
The Tec family tyrosine kinases Itk and Rlk regulate the development of conventional CD8+ T cells.
Atherly LO, Lucas JA, Felices M, Yin CC, Reiner SL, Berg LJ
Immunity 2006 Jul;25(1):79-91
Immunity 2006 Jul;25(1):79-91
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 1 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 1 µg/mL of Anti-ITK Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
