AF740

antibody from Novus Biologicals

Targeting: CD80 B7-1, B7.1, CD28LG, CD28LG1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF740

Provider

Novus Biologicals

Product name

Goat Polyclonal B7-1/CD80 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse B7-1/CD80 in ELISAs and Western blots.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Frontline Science: High fat diet and leptin promote tumor progression by inducing myeloid-derived suppressor cells.

Clements VK, Long T, Long R, Figley C, Smith DMC, Ostrand-Rosenberg S
Journal of leukocyte biology 2018 Mar;103(3):395-407

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Aging is associated with increased regulatory T-cell function.

Garg SK, Delaney C, Toubai T, Ghosh A, Reddy P, Banerjee R, Yung R
Aging cell 2014 Jun;13(3):441-8

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Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.

Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
European journal of immunology 2009 Jan;39(1):85-95

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Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract.

He H, Li W, Chen SY, Zhang S, Chen YT, Hayashida Y, Zhu YT, Tseng SC
Investigative ophthalmology & visual science 2008 Oct;49(10):4468-75

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse B7-1/CD80 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7-1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of B7-1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either treated with 200 ng/mL LPS (filled histogram) or unstimulated (open histogram) were stained with Goat Anti-Mouse B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

IL-2 secretion Induced by B7-1/CD80 and Neutralization by Mouse B7-1/CD80 Antibody. Recombinant Mouse B7-1/CD80 Fc Chimera (Catalog # 740-B1) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Mouse B7-1/CD80 Fc Chimera (0.1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740). The ND₅₀ is typically 0.15-0.6 µg/mL.