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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
- Blocking/Neutralizing [1]
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- Product number
- MAB140-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human B7-1/CD80 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human B7-1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P33681- Isotype
- IgG
- Antibody clone number
- 37711
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The utility of urinary CD80 as a diagnostic marker in patients with renal diseases.
Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord.
Endothelial extracellular vesicles modulate the macrophage phenotype: Potential implications in atherosclerosis.
Tubular B7-1 expression parallels proteinuria levels, but not clinical outcomes in adult minimal change disease patients.
Autoantibodies against CD80 in patients with COPD.
Tumor-activated TCRγδ⁺ T cells from gastric cancer patients induce the antitumor immune response of TCRαβ⁺ T cells via their antigen-presenting cell-like effects.
ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
B7-CD28 costimulatory signals control the survival and proliferation of murine and human γδ T cells via IL-2 production.
Minamikawa S, Nozu K, Maeta S, Yamamura T, Nakanishi K, Fujimura J, Horinouchi T, Nagano C, Sakakibara N, Nagase H, Shima H, Noda K, Ninchoji T, Kaito H, Iijima K
Scientific reports 2018 Nov 23;8(1):17322
Scientific reports 2018 Nov 23;8(1):17322
Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord.
Li J, Xu SQ, Zhao YM, Yu S, Ge LH, Xu BH
Molecular medicine reports 2018 Dec;18(6):4969-4977
Molecular medicine reports 2018 Dec;18(6):4969-4977
Endothelial extracellular vesicles modulate the macrophage phenotype: Potential implications in atherosclerosis.
He S, Wu C, Xiao J, Li D, Sun Z, Li M
Scandinavian journal of immunology 2018 Apr;87(4):e12648
Scandinavian journal of immunology 2018 Apr;87(4):e12648
Tubular B7-1 expression parallels proteinuria levels, but not clinical outcomes in adult minimal change disease patients.
Lee SW, Baek SH, Paik JH, Kim S, Na KY, Chae DW, Chin HJ
Scientific reports 2017 Feb 2;7:41859
Scientific reports 2017 Feb 2;7:41859
Autoantibodies against CD80 in patients with COPD.
Luo XM, Liu XY, Tang JH, Yang W, Ni ZH, Chen QG, Wang X
Clinical & translational immunology 2016 Oct;5(10):e103
Clinical & translational immunology 2016 Oct;5(10):e103
Tumor-activated TCRγδ⁺ T cells from gastric cancer patients induce the antitumor immune response of TCRαβ⁺ T cells via their antigen-presenting cell-like effects.
Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
Journal of immunology research 2014;2014:593562
Journal of immunology research 2014;2014:593562
ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
Weissmüller S, Semmler LY, Kalinke U, Christians S, Müller-Berghaus J, Waibler Z
Blood 2012 Jun 28;119(26):6268-77
Blood 2012 Jun 28;119(26):6268-77
B7-CD28 costimulatory signals control the survival and proliferation of murine and human γδ T cells via IL-2 production.
Ribot JC, Debarros A, Mancio-Silva L, Pamplona A, Silva-Santos B
Journal of immunology (Baltimore, Md. : 1950) 2012 Aug 1;189(3):1202-8
Journal of immunology (Baltimore, Md. : 1950) 2012 Aug 1;189(3):1202-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- B7-1/CD80 in Human Tonsil. B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of B7-1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of B7-1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-2 secretion Induced by B7-1/CD80 and Neutralization by Human B7-1/CD80 Antibody. Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Human B7-1/CD80 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140). The ND50 is typically 0.2-1 µg/mL.
