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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- PA5-85913 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD80 (B7-1) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Tumor-Associated Macrophages in Vestibular Schwannoma and Relationship to Hearing.
Nisenbaum E, Misztal C, Szczupak M, Thielhelm T, Peña S, Mei C, Goncalves S, Bracho O, Ma R, Ivan ME, Morcos J, Telischi F, Liu XZ, Fernandez-Valle C, Dinh CT
OTO open 2021 Oct-Dec;5(4):2473974X211059111
OTO open 2021 Oct-Dec;5(4):2473974X211059111
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD80 (B7-1) in human brain tissue lysate using a CD80 (B7-1) Polyclonal antibody (Product # PA5-85913) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CD80 (B7-1) in N2A cells using a CD80 (B7-1) Polyclonal antibody (Product # PA5-85913) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CD80 (B7-1) in SHG-44 cells using a CD80 (B7-1) Polyclonal antibody (Product # PA5-85913) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD80 (B7-1) of paraffin-embedded Human tonsil tissue using a CD80-B7-1 Polyclonal antibody (Product #PA5-85913). Counter stained with hematoxylin..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD80 (B7-1) of paraffin-embedded Mouse lung tissue using a CD80-B7-1 Polyclonal antibody (Product #PA5-85913). Counter stained with hematoxylin..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD80 (B7-1) of paraffin-embedded Mouse spleen tissue using a CD80-B7-1 Polyclonal antibody (Product #PA5-85913). Counter stained with hematoxylin..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of CD80 (B7-1) in Hela cells using a CD80 (B7-1) Polyclonal Antibody (Product # PA5-85913) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Transwell cocultures. Confocal images show monocytes migrated toward vestibular schwannoma (VS) cells associated with serviceable and unserviceable hearing and polarized to M1-type CD80 + , M2-type CD163 + , or mixed-phenotype macrophages.
