Explore
Validate
Learn
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-15512 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD80 Monoclonal Antibody (2A2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15512 targets CD80 in indirect ELISA, IF and IHC applications and shows reactivity with Human samples. The MA5-15512 immunogen is purified recombinant fragment of CD80 expressed in E. Coli. MA5-15512 detects CD80 which has a predicted molecular weight of approximately 55kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A2
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Correction of arginine metabolism with sepiapterin-the precursor of nitric oxide synthase cofactor BH(4)-induces immunostimulatory-shift of breast cancer.
The protective effects of MSC-EXO against pulmonary hypertension through regulating Wnt5a/BMP signalling pathway.
Zheng X, Fernando V, Sharma V, Walia Y, Letson J, Furuta S
Biochemical pharmacology 2020 Jun;176:113887
Biochemical pharmacology 2020 Jun;176:113887
The protective effects of MSC-EXO against pulmonary hypertension through regulating Wnt5a/BMP signalling pathway.
Zhang Z, Ge L, Zhang S, Wang J, Jiang W, Xin Q, Luan Y
Journal of cellular and molecular medicine 2020 Dec;24(23):13938-13948
Journal of cellular and molecular medicine 2020 Dec;24(23):13938-13948
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of BCBL-1 cells using CD80/LAB7 monoclonal antibody (Product # MA5-15512) (Green). Blue: DRAQ5 fluorescent DNA dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human tonsil tissues using CD80/LAB7 monoclonal antibody (Product # MA5-15512).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7. Sepiapterin reprograms M2-type TAMs to M1-type TAMs in mammary tumors. A) (Left) Mammary tumors from MMTV-PyMT mice were ex vivo- cultured as in Fig. 5A , paraffin-embedded, sectioned and co-stained for M1/M2 macrophage markers: CD80 (M1, red) vs. CD163 (M2, green). Counter-stained with DAPI. Note the prominent M2-type macrophages (green) in control and L-NAME-treated tumors vs. prominent M1-type macrophages (red) in SEP-treated tumor. Scale bars: 50 mum. (Right) Quantification of the intensity of CD80 or CD163 signal per cell. Error bars: +- SEM. *, p-value < 0.05, ***, p-value < 0.001. B) Scheme of signaling pathways ivolved in sepiapterin-mediated immuno-stimulatory shift of macrophage and cancer cell.
