Antibody data
- Antibody Data
- Antigen structure
- References [43]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 19483-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#19483-1-AP, RRID:AB_10644440
- Product name
- Nestin antibody
- Antibody type
- Polyclonal
- Description
- Nestin antibody (Cat. #19483-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Regulatory T cells promote glioma cell stemness through TGF-β-NF-κB-IL6-STAT3 signaling.
The type 3 adenylyl cyclase is crucial for intestinal mucosal neural network in the gut lamina propria.
miR-221/222 suppression induced by activation of the cAMP/PKA/CREB1 pathway is required for cAMP-induced bidirectional differentiation of glioma cells.
Effect of combination therapy with neural stem cell transplantation and teramethylpyrazine in rats following acute spinal cord injury.
BDNF-overexpressing human umbilical cord mesenchymal stem cell-derived motor neurons improve motor function and prolong survival in amyotrophic lateral sclerosis mice.
Effect of the interference with DRP1 expression on the biological characteristics of glioma stem cells.
The Construction and Analysis of ceRNA Network and Patterns of Immune Infiltration in Colon Adenocarcinoma Metastasis.
Tumor Necrosis Factor-α Regulates the TRPA1 Expression in Human Odontoblast-Like Cells.
Exosomes from patients with major depression cause depressive-like behaviors in mice with involvement of miR-139-5p-regulated neurogenesis.
Artesunate promotes the proliferation of neural stem/progenitor cells and alleviates Ischemia-reperfusion Injury through PI3K/Akt/FOXO-3a/p27kip1 signaling pathway.
Dose-dependent effects of tetramethylpyrazine on the characteristics of human umbilical cord mesenchymal stem cells for stroke therapy.
Ghrelin promotes neural differentiation of adipose tissue-derived mesenchymal stem cell via AKT/mTOR and β-catenin signaling pathways.
α7nAchR mediates transcutaneous auricular vagus nerve stimulation-induced neuroprotection in a rat model of ischemic stroke by enhancing axonal plasticity.
Zinc Promotes Patient-Derived Induced Pluripotent Stem Cell Neural Differentiation via ERK-STAT Signaling.
Transplantation of Nurr1-overexpressing neural stem cells and microglia for treating parkinsonian rats.
The Interleukin-33/ST2 axis promotes glioma mesenchymal transition, stemness and TMZ resistance via JNK activation.
Dynamic analysis of 4E-BP1 phosphorylation in neurons with Tsc2 or Depdc5 knockout.
TRIM24 promotes stemness and invasiveness of glioblastoma cells via activating Sox2 expression.
Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme.
Catalpol Enhances Neurogenesis And Inhibits Apoptosis Of New Neurons Via BDNF, But Not The BDNF/Trkb Pathway.
Long non-coding RNA linc00645 promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma.
circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma.
Constraint-induced movement therapy enhances angiogenesis and neurogenesis after cerebral ischemia/reperfusion.
Nell-1 promotes the neural-like differentiation of dental pulp cells.
Brain-derived neurotrophic factor modified human umbilical cord mesenchymal stem cells-derived cholinergic-like neurons improve spatial learning and memory ability in Alzheimer's disease rats.
Generation and Characterization of Induced Pluripotent Stem Cells from Mononuclear Cells in Schizophrenic Patients.
Toxicity mechanism of sevoflurane in neural stem cells of rats through DNA methylation.
QKI deficiency maintains glioma stem cell stemness by activating the SHH/GLI1 signaling pathway.
Mild thermotherapy and hyperbaric oxygen enhance sensitivity of TMZ/PSi nanoparticles via decreasing the stemness in glioma.
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.
Comparative proteomic analysis of mouse models of pathological and physiological cardiac hypertrophy, with selection of biomarkers of pathological hypertrophy by integrative Proteogenomics.
Protein Kinase A/CREB Signaling Prevents Adriamycin-Induced Podocyte Apoptosis via Upregulation of Mitochondrial Respiratory Chain Complexes.
In Vitro Comparison of 2D-Cell Culture and 3D-Cell Sheets of Scleraxis-Programmed Bone Marrow Derived Mesenchymal Stem Cells to Primary Tendon Stem/Progenitor Cells for Tendon Repair.
MicroRNA-326 sensitizes human glioblastoma cells to curcumin via the SHH/GLI1 signaling pathway.
Expression of Cannabinoid Type 1 Receptors in Human Odontoblast Cells.
Integrin endocytosis on elastic substrates mediates mechanosensing.
Mechanical stimulation of human tendon stem/progenitor cells results in upregulation of matrix proteins, integrins and MMPs, and activation of p38 and ERK1/2 kinases.
Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.
The failure in the stabilization of glioblastoma-derived cell lines: spontaneous in vitro senescence as the main culprit.
High-mobility group box 1 released from astrocytes promotes the proliferation of cultured neural stem/progenitor cells.
Spontaneous in vitro senescence of glioma cells confirmed by an antibody against IDH1R132H.
Oxygen glucose deprivation/reperfusion astrocytes promotes primary neural stem/progenitor cell proliferation by releasing high-mobility group box 1.
Malignant Leydig cell tumor with spindle-shaped cells in a male CD-1 mouse.
Liu S, Zhang C, Wang B, Zhang H, Qin G, Li C, Cao L, Gao Q, Ping Y, Zhang K, Lian J, Zhao Q, Wang D, Zhang Z, Zhao X, Yang L, Huang L, Yang B, Zhang Y
Cancer immunology, immunotherapy : CII 2021 Sep;70(9):2601-2616
Cancer immunology, immunotherapy : CII 2021 Sep;70(9):2601-2616
The type 3 adenylyl cyclase is crucial for intestinal mucosal neural network in the gut lamina propria.
Zhou K, Zhou Y, Yang D, Chen T, Liu X, Li S, Wang Z
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2021 Sep;33(9):e14140
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2021 Sep;33(9):e14140
miR-221/222 suppression induced by activation of the cAMP/PKA/CREB1 pathway is required for cAMP-induced bidirectional differentiation of glioma cells.
Liu Q, Tian R, Yu P, Shu M
FEBS letters 2021 Nov;595(22):2829-2843
FEBS letters 2021 Nov;595(22):2829-2843
Effect of combination therapy with neural stem cell transplantation and teramethylpyrazine in rats following acute spinal cord injury.
Zhang H, Huang Z, Guo M, Meng L, Piao M, Zhang M, Yu H
Neuroreport 2021 Nov 2;32(16):1311-1319
Neuroreport 2021 Nov 2;32(16):1311-1319
BDNF-overexpressing human umbilical cord mesenchymal stem cell-derived motor neurons improve motor function and prolong survival in amyotrophic lateral sclerosis mice.
Wang J, Hu W, Feng Z, Feng M
Neurological research 2021 Mar;43(3):199-209
Neurological research 2021 Mar;43(3):199-209
Effect of the interference with DRP1 expression on the biological characteristics of glioma stem cells.
Zhang L, Cao H, Tao H, Yang J, Gong W, Hu Q
Experimental and therapeutic medicine 2021 Jul;22(1):696
Experimental and therapeutic medicine 2021 Jul;22(1):696
The Construction and Analysis of ceRNA Network and Patterns of Immune Infiltration in Colon Adenocarcinoma Metastasis.
Chang Z, Huang R, Fu W, Li J, Ji G, Huang J, Shi W, Yin H, Wang W, Meng T, Huang Z, Wei Q, Qin H
Frontiers in cell and developmental biology 2020;8:688
Frontiers in cell and developmental biology 2020;8:688
Tumor Necrosis Factor-α Regulates the TRPA1 Expression in Human Odontoblast-Like Cells.
Liu J, Que K, Liu Y, Zang C, Wen J
Journal of pain research 2020;13:1655-1664
Journal of pain research 2020;13:1655-1664
Exosomes from patients with major depression cause depressive-like behaviors in mice with involvement of miR-139-5p-regulated neurogenesis.
Wei ZX, Xie GJ, Mao X, Zou XP, Liao YJ, Liu QS, Wang H, Cheng Y
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2020 May;45(6):1050-1058
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2020 May;45(6):1050-1058
Artesunate promotes the proliferation of neural stem/progenitor cells and alleviates Ischemia-reperfusion Injury through PI3K/Akt/FOXO-3a/p27kip1 signaling pathway.
Zhang K, Yang Y, Ge H, Wang J, Chen X, Lei X, Zhong J, Zhang C, Xian J, Lu Y, Tan L, Feng H
Aging 2020 May 7;12(9):8029-8048
Aging 2020 May 7;12(9):8029-8048
Dose-dependent effects of tetramethylpyrazine on the characteristics of human umbilical cord mesenchymal stem cells for stroke therapy.
Cao H, Zhu X, Zhang J, Xu M, Ge L, Zhang C
Neuroscience letters 2020 Mar 23;722:134797
Neuroscience letters 2020 Mar 23;722:134797
Ghrelin promotes neural differentiation of adipose tissue-derived mesenchymal stem cell via AKT/mTOR and β-catenin signaling pathways.
Liu GB, Pan YM, Liu YS, Hu JH, Zhang XD, Zhang DW, Wang Y, Feng YK, Yu JB, Cheng YX
The Kaohsiung journal of medical sciences 2020 Jun;36(6):405-416
The Kaohsiung journal of medical sciences 2020 Jun;36(6):405-416
α7nAchR mediates transcutaneous auricular vagus nerve stimulation-induced neuroprotection in a rat model of ischemic stroke by enhancing axonal plasticity.
Li J, Zhang Q, Li S, Niu L, Ma J, Wen L, Zhang L, Li C
Neuroscience letters 2020 Jun 21;730:135031
Neuroscience letters 2020 Jun 21;730:135031
Zinc Promotes Patient-Derived Induced Pluripotent Stem Cell Neural Differentiation via ERK-STAT Signaling.
Yang M, Bao D, Shi A, Yuan H, Wang J, He W, Tong X, Qin H
Stem cells and development 2020 Jul 1;29(13):863-875
Stem cells and development 2020 Jul 1;29(13):863-875
Transplantation of Nurr1-overexpressing neural stem cells and microglia for treating parkinsonian rats.
Qian Y, Chen XX, Wang W, Li JJ, Wang XP, Tang ZW, Xu JT, Lin H, Yang ZY, Li LY, Song XB, Guo JZ, Bian LG, Zhou L, Lu D, Deng XL
CNS neuroscience & therapeutics 2020 Jan;26(1):55-65
CNS neuroscience & therapeutics 2020 Jan;26(1):55-65
The Interleukin-33/ST2 axis promotes glioma mesenchymal transition, stemness and TMZ resistance via JNK activation.
Lin L, Li Y, Liu M, Li Q, Liu Q, Li R
Aging 2020 Jan 29;12(2):1685-1703
Aging 2020 Jan 29;12(2):1685-1703
Dynamic analysis of 4E-BP1 phosphorylation in neurons with Tsc2 or Depdc5 knockout.
Iffland PH 2nd, Barnes AE, Baybis M, Crino PB
Experimental neurology 2020 Dec;334:113432
Experimental neurology 2020 Dec;334:113432
TRIM24 promotes stemness and invasiveness of glioblastoma cells via activating Sox2 expression.
Zhang LH, Yin YH, Chen HZ, Feng SY, Liu JL, Chen L, Fu WL, Sun GC, Yu XG, Xu DG
Neuro-oncology 2020 Dec 18;22(12):1797-1808
Neuro-oncology 2020 Dec 18;22(12):1797-1808
Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme.
Chen Y, Liu P, Sun P, Jiang J, Zhu Y, Dong T, Cui Y, Tian Y, An T, Zhang J, Li Z, Yang X
Theranostics 2019;9(5):1453-1473
Theranostics 2019;9(5):1453-1473
Catalpol Enhances Neurogenesis And Inhibits Apoptosis Of New Neurons Via BDNF, But Not The BDNF/Trkb Pathway.
Zhu HF, Shao Y, Qin L, Wang JH, Feng S, Jiang YB, Wan D
Drug design, development and therapy 2019;13:4145-4157
Drug design, development and therapy 2019;13:4145-4157
Long non-coding RNA linc00645 promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma.
Li C, Zheng H, Hou W, Bao H, Xiong J, Che W, Gu Y, Sun H, Liang P
Cell death & disease 2019 Sep 26;10(10):717
Cell death & disease 2019 Sep 26;10(10):717
circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma.
Chen J, Chen T, Zhu Y, Li Y, Zhang Y, Wang Y, Li X, Xie X, Wang J, Huang M, Sun X, Ke Y
Journal of experimental & clinical cancer research : CR 2019 Sep 11;38(1):398
Journal of experimental & clinical cancer research : CR 2019 Sep 11;38(1):398
Constraint-induced movement therapy enhances angiogenesis and neurogenesis after cerebral ischemia/reperfusion.
Zhai ZY, Feng J
Neural regeneration research 2019 Oct;14(10):1743-1754
Neural regeneration research 2019 Oct;14(10):1743-1754
Nell-1 promotes the neural-like differentiation of dental pulp cells.
Han Q, Wang Q, Wu J, Li M, Fang Y, Zhu H, Wang X
Biochemical and biophysical research communications 2019 May 28;513(2):515-521
Biochemical and biophysical research communications 2019 May 28;513(2):515-521
Brain-derived neurotrophic factor modified human umbilical cord mesenchymal stem cells-derived cholinergic-like neurons improve spatial learning and memory ability in Alzheimer's disease rats.
Hu W, Feng Z, Xu J, Jiang Z, Feng M
Brain research 2019 May 1;1710:61-73
Brain research 2019 May 1;1710:61-73
Generation and Characterization of Induced Pluripotent Stem Cells from Mononuclear Cells in Schizophrenic Patients.
Liu Q, Du J, Fan J, Li W, Guo W, Feng H, Lin J
Cell journal 2019 Jul;21(2):161-168
Cell journal 2019 Jul;21(2):161-168
Toxicity mechanism of sevoflurane in neural stem cells of rats through DNA methylation.
Wang K, Tian Y, Zhang Y, Li X, Wei X, Hu H, Xu S
Experimental and therapeutic medicine 2019 Jul;18(1):237-241
Experimental and therapeutic medicine 2019 Jul;18(1):237-241
QKI deficiency maintains glioma stem cell stemness by activating the SHH/GLI1 signaling pathway.
Han B, Wang R, Chen Y, Meng X, Wu P, Li Z, Duan C, Li Q, Li Y, Zhao S, Jiang C, Cai J
Cellular oncology (Dordrecht) 2019 Dec;42(6):801-813
Cellular oncology (Dordrecht) 2019 Dec;42(6):801-813
Mild thermotherapy and hyperbaric oxygen enhance sensitivity of TMZ/PSi nanoparticles via decreasing the stemness in glioma.
Zeng X, Wang Q, Tan X, Jia L, Li Y, Hu M, Zhang Z, Bai X, Zhu Y, Yang X
Journal of nanobiotechnology 2019 Apr 1;17(1):47
Journal of nanobiotechnology 2019 Apr 1;17(1):47
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.
Jia W, Jiang X, Liu W, Wang L, Zhu B, Zhu H, Liu X, Zhong M, Xie D, Huang W, Jia W, Li S, Liu X, Zuo X, Cheng D, Dai J, Ren C
International journal of oncology 2018 Jun;52(6):1787-1800
International journal of oncology 2018 Jun;52(6):1787-1800
Comparative proteomic analysis of mouse models of pathological and physiological cardiac hypertrophy, with selection of biomarkers of pathological hypertrophy by integrative Proteogenomics.
Kwon HK, Jeong H, Hwang D, Park ZY
Biochimica et biophysica acta. Proteins and proteomics 2018 Jul 23;
Biochimica et biophysica acta. Proteins and proteomics 2018 Jul 23;
Protein Kinase A/CREB Signaling Prevents Adriamycin-Induced Podocyte Apoptosis via Upregulation of Mitochondrial Respiratory Chain Complexes.
Xie K, Zhu M, Xiang P, Chen X, Kasimumali A, Lu R, Wang Q, Mou S, Ni Z, Gu L, Pang H
Molecular and cellular biology 2018 Jan 1;38(1)
Molecular and cellular biology 2018 Jan 1;38(1)
In Vitro Comparison of 2D-Cell Culture and 3D-Cell Sheets of Scleraxis-Programmed Bone Marrow Derived Mesenchymal Stem Cells to Primary Tendon Stem/Progenitor Cells for Tendon Repair.
Hsieh CF, Yan Z, Schumann RG, Milz S, Pfeifer CG, Schieker M, Docheva D
International journal of molecular sciences 2018 Aug 2;19(8)
International journal of molecular sciences 2018 Aug 2;19(8)
MicroRNA-326 sensitizes human glioblastoma cells to curcumin via the SHH/GLI1 signaling pathway.
Yin S, Du W, Wang F, Han B, Cui Y, Yang D, Chen H, Liu D, Liu X, Zhai X, Jiang C
Cancer biology & therapy 2018 Apr 3;19(4):260-270
Cancer biology & therapy 2018 Apr 3;19(4):260-270
Expression of Cannabinoid Type 1 Receptors in Human Odontoblast Cells.
Que K, He D, Jin Y, Wu L, Wang F, Zhao Z, Yang J, Deng J
Journal of endodontics 2017 Feb;43(2):283-288
Journal of endodontics 2017 Feb;43(2):283-288
Integrin endocytosis on elastic substrates mediates mechanosensing.
Li J, Chen H, Xu Y, Hu J, Xie FQ, Yang C
Journal of biomechanics 2016 Sep 6;49(13):2644-2654
Journal of biomechanics 2016 Sep 6;49(13):2644-2654
Mechanical stimulation of human tendon stem/progenitor cells results in upregulation of matrix proteins, integrins and MMPs, and activation of p38 and ERK1/2 kinases.
Popov C, Burggraf M, Kreja L, Ignatius A, Schieker M, Docheva D
BMC molecular biology 2015 Mar 13;16:6
BMC molecular biology 2015 Mar 13;16:6
Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.
Alberton P, Dex S, Popov C, Shukunami C, Schieker M, Docheva D
Stem cells and development 2015 Mar 1;24(5):597-609
Stem cells and development 2015 Mar 1;24(5):597-609
The failure in the stabilization of glioblastoma-derived cell lines: spontaneous in vitro senescence as the main culprit.
Stoczynska-Fidelus E, Piaskowski S, Bienkowski M, Banaszczyk M, Hulas-Bigoszewska K, Winiecka-Klimek M, Radomiak-Zaluska A, Och W, Borowiec M, Zieba J, Treda C, Rieske P
PloS one 2014;9(1):e87136
PloS one 2014;9(1):e87136
High-mobility group box 1 released from astrocytes promotes the proliferation of cultured neural stem/progenitor cells.
Li M, Sun L, Luo Y, Xie C, Pang Y, Li Y
International journal of molecular medicine 2014 Sep;34(3):705-14
International journal of molecular medicine 2014 Sep;34(3):705-14
Spontaneous in vitro senescence of glioma cells confirmed by an antibody against IDH1R132H.
Stoczynska-Fidelus E, Och W, Rieske P, Bienkowski M, Banaszczyk M, Winiecka-Klimek M, Zieba J, Janik K, Rosiak K, Treda C, Stawski R, Radomiak-Zaluska A, Piaskowski S
Anticancer research 2014 Jun;34(6):2859-67
Anticancer research 2014 Jun;34(6):2859-67
Oxygen glucose deprivation/reperfusion astrocytes promotes primary neural stem/progenitor cell proliferation by releasing high-mobility group box 1.
Li M, Sun L, Li Y, Xie C, Wan D, Luo Y
Neurochemical research 2014 Aug;39(8):1440-50
Neurochemical research 2014 Aug;39(8):1440-50
Malignant Leydig cell tumor with spindle-shaped cells in a male CD-1 mouse.
Ohnuma A, Yoshida T, Takahashi N, Akema S, Kumagai M, Chiba Y, Kashimoto Y, Kuwahara M, Nakashima N, Harada T
The Journal of veterinary medical science 2010 May;72(5):661-4
The Journal of veterinary medical science 2010 May;72(5):661-4
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- rat brain tissue were subjected to SDS PAGE followed by western blot with 19483-1-AP(NES antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using NES antibody19483-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 19483-1-AP(NES antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 19483-1-AP(NES antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue