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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- OAAB01332 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- NES Antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of Nestin Antibody (S1409) in HepG2 cell lysates (35ug/lane). NES (arrow) was detected using the purified Pab.
- Sample type
- HepG2 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using Nestin Antibody (S1409)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- Flow cytometric analysis ofHepG2 cells using Nestin Antibody (S1409)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Nestin Antibody (S1409), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Sample type
- human hepatocarcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
