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- Antibody Data
- Antigen structure
- References [7]
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- Immunocytochemistry [3]
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- Product number
- AF2736 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat Nestin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
EDM00749- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Conditional Inactivation of Pen-2 in the Developing Neocortex Leads to Rapid Switch of Apical Progenitors to Basal Progenitors.
Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortex.
Nestin-positive microglia in adult rat cerebral cortex.
Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesis.
Mechanisms of murine lacrimal gland repair after experimentally induced inflammation.
Vasopressin mediates mitogenic responses to adrenalectomy in the rat anterior pituitary.
Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
Cheng S, Liu T, Hu Y, Xia Y, Hou J, Huang C, Zou X, Liang J, Stone Shi Y, Zheng Y, Lu J, Chen G
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Mar 20;39(12):2195-2207
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Mar 20;39(12):2195-2207
Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortex.
Takamori Y, Hirahara Y, Wakabayashi T, Mori T, Koike T, Kataoka Y, Tamura Y, Kurebayashi S, Kurokawa K, Yamada H
IBRO reports 2018 Dec;5:99-109
IBRO reports 2018 Dec;5:99-109
Nestin-positive microglia in adult rat cerebral cortex.
Takamori Y, Mori T, Wakabayashi T, Nagasaka Y, Matsuzaki T, Yamada H
Brain research 2009 May 13;1270:10-8
Brain research 2009 May 13;1270:10-8
Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesis.
Gregorian C, Nakashima J, Le Belle J, Ohab J, Kim R, Liu A, Smith KB, Groszer M, Garcia AD, Sofroniew MV, Carmichael ST, Kornblum HI, Liu X, Wu H
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Feb 11;29(6):1874-86
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Feb 11;29(6):1874-86
Mechanisms of murine lacrimal gland repair after experimentally induced inflammation.
Zoukhri D, Fix A, Alroy J, Kublin CL
Investigative ophthalmology & visual science 2008 Oct;49(10):4399-406
Investigative ophthalmology & visual science 2008 Oct;49(10):4399-406
Vasopressin mediates mitogenic responses to adrenalectomy in the rat anterior pituitary.
Subburaju S, Aguilera G
Endocrinology 2007 Jul;148(7):3102-10
Endocrinology 2007 Jul;148(7):3102-10
Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
Biotechnology and bioengineering 2007 Aug 15;97(6):1617-25
Biotechnology and bioengineering 2007 Aug 15;97(6):1617-25
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- beta-III Tubulin and Nestin in Rat Cortical Stem Cells. beta-III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta-III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195) and 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
